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dsred rab11 wt  (Addgene inc)


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    Addgene inc dsred rab11 wt
    Dsred Rab11 Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dsred rab11 wt/product/Addgene inc
    Average 93 stars, based on 53 article reviews
    dsred rab11 wt - by Bioz Stars, 2026-06
    93/100 stars

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    Role of the WIPI2 FSSS motif for recruiting WIPI2 and Retriever to Rab11 endosomes . WIPI2-knockdown HK2 cells were transfected with plasmids expressing the indicated siRNA- resistant EGFP WIPI2 variants and mCherry-RAB5, mCherry-RAB7, or DsRed-Rab11 were fixed, permeabilized, immuno-stained for VPS35L and analyzed by confocal microscopy. Examples of the quantified images are presented in Supplementary Figures S5 to S7. Colocalization with EGFP WIPI2 and VPS35L was assessed for: A. RAB11 B. RAB5 C. RAB7. Colocalization was quantified using Manders’ colocalization coefficient M2, calculated in ImageJ. M2 refers to the fraction of VPS35L colocalising with the different Rab-proteins. For the triple colocalizations WIPI2/VPS35L/RAB M2 indicates the fraction of green pixels (WIPI2) overlapping with the pixels positive for the VPS35L/RAB colocalization. Colocalization was quantified from three independent experiments in a total of 120 cells. P values were calculated applying Welch’s t-test with unequal variances. The analysis was performed with 99% confidence. ***P < 0.0001.

    Journal: bioRxiv

    Article Title: CROP2, a Retriever-PROPPIN Complex Mediating Protein Export from Endosomes to the Plasma Membrane

    doi: 10.1101/2025.10.08.681146

    Figure Lengend Snippet: Role of the WIPI2 FSSS motif for recruiting WIPI2 and Retriever to Rab11 endosomes . WIPI2-knockdown HK2 cells were transfected with plasmids expressing the indicated siRNA- resistant EGFP WIPI2 variants and mCherry-RAB5, mCherry-RAB7, or DsRed-Rab11 were fixed, permeabilized, immuno-stained for VPS35L and analyzed by confocal microscopy. Examples of the quantified images are presented in Supplementary Figures S5 to S7. Colocalization with EGFP WIPI2 and VPS35L was assessed for: A. RAB11 B. RAB5 C. RAB7. Colocalization was quantified using Manders’ colocalization coefficient M2, calculated in ImageJ. M2 refers to the fraction of VPS35L colocalising with the different Rab-proteins. For the triple colocalizations WIPI2/VPS35L/RAB M2 indicates the fraction of green pixels (WIPI2) overlapping with the pixels positive for the VPS35L/RAB colocalization. Colocalization was quantified from three independent experiments in a total of 120 cells. P values were calculated applying Welch’s t-test with unequal variances. The analysis was performed with 99% confidence. ***P < 0.0001.

    Article Snippet: Vectors expressing tagged RAB proteins were purchased from Addgene: DsRed- RAB11 (12679; deposited by R. Pagano); mCherry-RAB7 (61804; deposited by G. Voeltz); mCherry-RAB5 (49201; deposited by G. Voeltz).

    Techniques: Knockdown, Transfection, Expressing, Staining, Confocal Microscopy